Coding

Part:BBa_K4382007:Design

Designed by: Ashwin Ananthanarayanan   Group: iGEM22_IISER_Bhopal   (2022-10-10)


Pectin lyase - PelA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 570
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 674
    Illegal BsaI site found at 1048
    Illegal BsaI site found at 1153


Design Notes

The gene is codon optimized for cloning into Bacillus subtilis 168.



Source

This gene is sourced from the soil bacterium Bacillus subtilis 7-3-3

References